{ Vitamin D Test Kit }

  • Accurate 25-OH Vitamin D Assessment with Poclight CLIA Assays

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    1. Why 25-OH Vitamin D Matters

     

    The main physiological role of vitamin D and its metabolites is to maintain normal blood calcium and phosphorus levels, to ensure bone health and normal neuromuscular function.

     

    25-Hydroxy Vitamin D is the main storage form of vitamin D in the human body, and the detection of serum 25-Hydroxy VD level has been recognized as the most reasonable indicator to reflect the status of vitamin D.

     

    Clinically, 25-hydroxyvitamin D is the preferred indicator because:

    ● It reflects combined effects of sunlight, diet, and supplements

    ● It is stable in serum and plasma

    ● It reliably shows long-term vitamin D reserves

     

    25 OH Vitamin D

     

    Inadequate vitamin D can contribute to:

    ● Reduced bone density and greater fracture risk

    ● Muscle weakness and higher fall likelihood

    ● Impaired immune function

    ● Seasonal fatigue or malaise

     

    2.  Reference Value for Vitamin D

     

    Levels of 50 nmol/L (20 ng/mL) or more are sufficient for most people.

    Optimal serum concentrations of 25(OH)D for bone and general health have not been established because they are likely to vary by stage of life, by race and ethnicity, and with each physiological measure used.

     

    VD REF

     

    In addition, although 25(OH)D levels rise in response to increased vitamin D intake, the relationship is nonlinear. The amount of increase varies, for example, by baseline serum levels and duration of supplementation.

     

    Understanding these dynamics highlights the importance of accurate, reliable measurement of 25(OH)D, precisely what our Poclight 25-OH VD assay is designed to provide.

     

    3.  Poclight 25-OH VD Assay Key Features

     

    1. LOD: 3ng/mL
    2. Linearity: 3-70ng/mL, in this linear range, the linear correlation coefficient "r" is not less than 0.9900
    3. Repeatability: CV<5% 
    4. Exceptional Speed: Reduces the detection time from 3 hours to just 10 minutes.
    5. Compatible with Poclight C5000 Benchtop CLIA Analyzer

     

    Operation process

    VD OPERATION

     

    If you’d like to have our products in your lab or distribution lineup, feel free to contact us to discuss pricing, partnership, and next steps.

     

  • How to Choose the Right Vitamin D Assay for Your Lab?

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    Vitamin D plays a critical role in maintaining bone health, supporting immune function, and reducing inflammation. With growing awareness of vitamin D deficiency and its health implications, more people are seeking ways to accurately check their vitamin D levels.

     

    1. Why Test for Vitamin D?

    • Vitamin D deficiency is surprisingly common, especially in individuals who:
    • Spend limited time outdoors
    • Live in areas with limited sunlight
    • Follow vegan diets or have digestive conditions
    • Are elderly, obese, or have darker skin
    • A deficiency in vitamin D can lead to:
    • Osteomalacia or rickets (soft bones)
    • Increased risk of fractures
    • Weakened immune response
    • Muscle weakness and fatigue

    Routine testing is essential for at-risk individuals or anyone wanting to optimize their long-term health.

     

    Vitamin D application

    2. Common Types of Vitamin D Tests

    There are two primary forms of vitamin D that can be measured in the blood:

    Test Name

    What It Measures

    Pros

    Cons

    25-hydroxyvitamin D [25(OH)VD]

    The main circulating form

    Gold standard for assessing deficiency

    May not reflect real-time biological activity

     

    1,25-dihydroxyvitamin D [1,25(OH)₂D]

    The active hormone form

    Useful in specific diseases (kidney issues)

    Not recommended for general screening

    Among these, 25(OH)VD is the preferred and most reliable marker for evaluating vitamin D status.

     

    3. Testing Technologies: Which Is Most Accurate?

    Vitamin D levels can be tested using various technologies:

    Technologies

    Principle:

    Pros

    Cons

     Immunoassays (e.g., CLIA – Chemiluminescence Immunoassay)

    Detects 25(OH)VD through a highly sensitive reaction between antigens and antibodies.

     

    Fast, automated, cost-effective, widely used in clinical laboratories.

    Conventional analyzers often require large spaces and significant investment.

    LC-MS/MS (Liquid Chromatography–Mass Spectrometry)

     

    Very accurate, can detect multiple forms of vitamin D.

    Expensive, requires specialized equipment and skilled technicians.

     Home Test Kits

     

    Convenient, no lab visit needed.

    Limited accuracy, inconsistent quality, often based on dried blood spot collection.

     

    Compared to other methods, CLIA-based 25(OH)VD testing stands out by offering the best combination of clinical accuracy, affordability, and accessibility.

    Poclight offer a compact and efficient chemiluminescence immunoassay system C5000 designed to provide rapid and accurate vitamin D results.

    Based on CRET technology (Chemiluminescence Resonance Energy Transfer), Poclight Delivers results within 10 minutes, ideal for point-of-care testing. Compact size: A4 footprint, weighs only 7kg, affordable for most clinics and laboratories; Lyophilized reagents, no cold chain required–easy global shipment;CE-marked and suitable for international OEM/ODM collaboration.Our system enables clinics, laboratories, and primary care facilities to perform reliable testing even in decentralized or resource-limited settings.

    If you're wondering which test is the most accurate for detecting vitamin D deficiency, the answer is clear:Test the 25(OH)VD level using a high-quality, standardized CLIA method.With Poclight’s chemiluminescence technology, you can trust the results–whether you're a healthcare provider or a distributor seeking advanced, compact diagnostic solutions.

     

    4. Want to Learn More?

    Contact us to learn how our vitamin D testing system can support your clinical or commercial needs.

    Download our product brochure or request a live demo.